NAD+ (Buffered) 1000 mg – Coenzyme for mitochondrial research

What is buffered NAD+ (1000 mg)?

NAD+ (nicotinamide adenine dinucleotide) is a key coenzyme present in every cell, involved in hundreds of metabolic reactions — above all in energy production (ATP) and DNA repair. The version offered by Peptology is buffered NAD+ 1000 mg — a stabilized, easily soluble form intended for scientific research applications on cellular energy, mitochondria and aging processes.

It is worth emphasizing: NAD+ is not a peptide, but a coenzyme. In the Peptology catalog, however, it belongs to the same research category as mitochondrial and regenerative peptides — peptides for energy and regeneration — because it addresses the same research area: the cell's energy metabolism and mitochondrial function.

A buffered formula means the pH has been stabilized — raw NAD+ is strongly acidic, and buffering reduces this acidity, improving the solubility and stability of the solution under laboratory conditions. It is a research compound — it is not a medicinal product and holds no registration for clinical use in the EU.

In the version offered by Peptology, NAD+ is supplied as a lyophilized powder of high purity, confirmed by independent laboratory analysis (CoA for every batch), at a dose of 1000 mg per vial. The product is intended exclusively for scientific research and in vitro applications.

How NAD+ works — research mechanisms

In the scientific literature, NAD+ is described as a central hub of cellular metabolism. Below are the three most frequently studied mechanisms.

Why NAD+ is a central coenzyme

The level of NAD+ in cells naturally declines with age, which makes it a frequent subject of research on aging and energy metabolism. NAD+ links three areas: energy production in mitochondria, DNA repair by PARP, and activation of sirtuins — which is why it is described as a hub connecting metabolism, regeneration and the cell's stress response.

In the area of mitochondrial research, NAD+ is sometimes paired with the peptide SS-31, which acts at the level of the mitochondrial membrane as an antioxidant. These data come from preclinical and in vitro studies and do not constitute a claim about the effect of the research-grade product.

Available format: buffered NAD+ 1000 mg

Buffered NAD+ is offered in a single lyophilized format — 1000 mg per vial. This is a high mass compared with typical peptides, covering both single in vitro series and longer cellular and animal protocols with higher research-material consumption.

The buffered 1000 mg format has confirmed high purity and a laboratory certificate (CoA) for the batch. The choice of solvent volume depends on the scale of the research protocol.

Pharmacokinetic profile (research framework)

NAD+ is an endogenous molecule with rapid metabolism and intracellular turnover. In a research context the following features are relevant:

• Nature: endogenous coenzyme — naturally present in every cell
• Form: buffered, with stabilized pH (improved solubility and solution stability)
• Observation peak: in data from intravenous protocols, maximum concentration was described around ~30 min after administration
• Decline with age: cellular NAD+ levels decrease with age — hence the interest of aging researchers
• Route of administration in protocols: subcutaneous or intravenous, according to the research protocol
• Product form: lyophilized powder for reconstitution

Comparison: NAD+ vs related regenerative compounds

NAD+ addresses cellular energy and metabolism at the coenzyme level. For orientation, below we compare it with two popular compounds from the same research category — the mitochondrial peptide SS-31 and the repair peptide BPC-157. They have different points of action and are often studied together.

These compounds do not compete but address different mechanisms — energy (NAD+), mitochondrial-antioxidant (SS-31) and repair (BPC-157). That is why they are sometimes combined in research.

Research areas for NAD+

NAD+ is one of the most intensively studied coenzymes in the sciences of metabolism and aging. Below are the most frequently explored directions — all at the research stage, without confirmed clinical indications.

Cellular energy and mitochondria

In research models, NAD+ is linked with improved mitochondrial function and increased ATP production. This is the main reason for interest in it in the context of metabolic fatigue and post-exercise regeneration.

Aging and sirtuins

As a cofactor of sirtuins (SIRT1–SIRT7), NAD+ is studied in the context of longevity, metabolic regulation and the cell's stress response. The decline of its level with age is one of the main hypotheses in the biology of aging.

Neuroprotection and metabolism

Research is ongoing into the role of NAD+ in neurological health, insulin sensitivity and protection against oxidative stress. It should be emphasized that these are research directions, not confirmed therapeutic applications — research-grade NAD+ is not a medicine for any condition.

Note: The information is presented for scientific and informational purposes and is based on preclinical literature and early observations. It does not constitute medical advice, a recommendation for use or a claim about the effect of the research-grade product offered by Peptology.

NAD+ profile at a glance

• Central coenzyme of metabolism — participates in ATP production and DNA repair
• Buffered form — stabilized pH, better solubility and solution stability
• Three main pathways: respiratory chain (ATP), PARP enzymes (DNA repair), sirtuins (SIRT1–7)
• A frequent subject of research on aging, mitochondria and energy metabolism
• Related compounds: the mitochondrial peptide SS-31 and the repair peptide BPC-157
• Every batch with a CoA and confirmed laboratory purity

All information provided concerns the research literature on NAD+ and should not be interpreted as a promise of effect, an instruction for use or a claim about the effect of the research-grade product offered by Peptology.

Research dosing protocols

In research protocols, NAD+ is usually administered after reconstitution of the lyophilizate:

• Subcutaneous or intravenous administration, according to the research protocol
• Because of the large mass (1000 mg/vial) it is especially important to precisely calculate concentration and volume
• Schedules are usually spread over time, with an adaptation phase taken into account (reactive effects tend to be stronger at the start)
• Maximum 3 mL of solvent per vial — use the peptide calculator for conversions

In combination protocols, NAD+ is sometimes paired with mitochondrial peptides (SS-31) or repair peptides (BPC-157, TB-500) to study effects at different levels of the cell. These schedules come from the research literature and do not constitute a recommendation for self-administration.

Side effects and safety

In research observations, NAD+ is described as well tolerated. Some of the reported effects are transient reactions that ease with adaptation. The data come mainly from early observations and intravenous protocols.

Most of the reported effects are mild and transient, and their intensity often depends on the rate of administration. This information is scientific in nature and does not constitute an instruction for use.

Storage and reconstitution

Buffered NAD+ is supplied as a lyophilized (freeze-dried) powder in a vial. In lyophilized form it is stable at room temperature, protected from light and moisture — refrigeration before reconstitution is not mandatory. After reconstitution, stability depends on the solvent used, with Peptide Matrix Water™ significantly extending the solution's lifespan.

• Before reconstitution: room temperature, in the original packaging, protected from light and moisture. A refrigerator is not mandatory.
• After reconstitution with Peptide Matrix Water™: stability in the refrigerator +2 °C to +8 °C for up to 60 days under appropriate laboratory conditions — the stabilizing formula extends the solution's lifespan relative to standard solvents.
• After reconstitution with bacteriostatic or sterile water: stability in the refrigerator +2 °C to +8 °C for up to 28 days under appropriate conditions. Do not refreeze the reconstituted solution.
• Reconstitution: in a laboratory environment, Peptide Matrix Water™, bacteriostatic water or sterile water for injection is usually used, depending on the protocol.
• Volume: maximum 3 mL of solvent (capacity of a standard vial). For a precise calculation of volume and units, use the peptide calculator.
• Homogenization: add the solvent slowly down the vial wall, without vigorous shaking, then wait 10–15 min. Swirl gently, do not shake.

Laboratory tip: With your order, you can add Peptide Matrix Water™ to the cart — a stabilizing solution designed for working with reconstituted research compounds. More in the article Bacteriostatic water vs acetic acid vs matrix water.

Frequently asked questions about NAD+

What exactly is NAD+ and is it a peptide?

NAD+ is a coenzyme (nicotinamide adenine dinucleotide), not a peptide. It participates in hundreds of metabolic reactions — mainly in energy production (ATP) and DNA repair. In the Peptology catalog it belongs to the energy and regeneration category because of its application area.

What does „buffered" mean?

Raw NAD+ is strongly acidic. Buffering stabilizes the pH, which improves the solubility and stability of the solution under laboratory conditions — hence the buffered form is more convenient to work with.

How does NAD+ differ from SS-31?

Both concern mitochondria, but at different levels. NAD+ is a coenzyme supporting energy production and metabolism; SS-31 is a peptide acting on the mitochondrial membrane as an antioxidant. In research they are sometimes combined as complementary.

Can NAD+ be combined with other compounds?

In the research literature, NAD+ is sometimes paired with mitochondrial peptides (SS-31) and repair peptides (BPC-157). These are research schedules, not recommendations for use.

How is NAD+ stored and reconstituted?

The lyophilized vial is stored at room temperature, protected from light and moisture. After reconstitution, stability depends on the solvent: with Peptide Matrix Water™ — up to 60 days in the refrigerator +2 °C to +8 °C; with bacteriostatic or sterile water — up to 28 days. Avoid refreezing.

Where to buy NAD+ for research?

Peptology offers buffered NAD+ 1000 mg as a research compound with confirmed purity and independent laboratory analysis for every batch. Fast shipping, discreet packaging and certificates of purity with the product. For scientific background, also see the article Peptides — what they are, how they work, main categories and safety profile.

Related products in the Peptology catalog

Researchers working with NAD+ often also consider related mitochondrial and regenerative compounds from the same category. Each of the products below has a certificate of purity:

See the full catalog: Peptides for energy and regeneration · Regeneration and anabolic peptides · Solutions for peptides · Blog on peptide research

Molecule identity

Laboratory reconstitution

Laboratory protocol — reconstitution, research protocols, storage

Standardized protocol applicable to all research peptides in the Peptology catalogue. To calculate the solution concentration (mg/mL) for each vial, use the peptide reconstitution calculator.

60-second protocol

Quick reference

What you need

Five rules for reconstitution

Calculating concentration

Storage

Four mistakes to avoid

Shipping information

🚚 Shipping information

• Products in our warehouse – orders placed by 15:00 on working days are dispatched the same day, later orders on the next working day.

• Delivery with GLS to your address in 3–5 working days.

• Shipping costs €5 and is free from €50.

Frequently Asked Questions (FAQ)

Is NAD+ suitable for neurodegenerative research?

Yes – it is actively investigated in conditions such as Parkinson's, Alzheimer's and multiple sclerosis.

In what form is NAD+ supplied and how should it be stored?

It is supplied as a lyophilized (freeze-dried) powder in a sealed vial. Store the unopened vial dry and protected from light, ideally at 2–8 °C. After laboratory reconstitution, keep the solution at 2–8 °C and use it within the period stated in the “Laboratory reconstitution” tab (up to 60 days with Peptide Matrix Water).

Is a prescription required?

No. The product is for laboratory and research use only.

Where can I buy NAD+ in Europe and what does it cost?

You can buy NAD+ in Europe online from Peptology, from 89,00 €. Delivery with GLS in 3–5 working days. Shipping is free from 50,00 €. Payment by card. Purity 99%+, with an HPLC report for every batch.

Laboratory Analysis

NAD+ (Buffered) - 1000 mg - Batch - 0226 - Purity