Peptide calculator: reconstitution and vial concentration

1. 1. Amount in vial (mg)
2. 2. Solvent added (mL)
3. 3. Amount per sample (mg)

Calculator information

Laboratory reconstitution calculator: enter the amount of peptide in the vial (mg) and the volume of solvent you added (mL) to get the concentration (mg/mL and µg/µL). Enter the amount each sample requires (mg) to calculate the solution volume in mL and µL. For laboratory research use only.

Quick conversions

• 1 mg = 1000 µg
• 1 mL = 1000 µL
• 1 mg/mL = 1 µg/µL

Reconstitution examples

10 mg vial + 2 mL solvent

Concentration 5 mg/mL (5 µg/µL). For 1 mg per sample: 0.2 mL (200 µL).

5 mg vial + 1 mL solvent

Concentration 5 mg/mL (5 µg/µL). For 0.5 mg per sample: 0.1 mL (100 µL).

Frequently asked questions

How are lyophilized peptides reconstituted?

Add the sterile solvent slowly along the wall of the vial. Do not shake: swirl gently and wait 10–15 minutes at room temperature until the solution is clear.

What is the difference between mg and µg?

1 mg = 1000 µg. The calculator gives the concentration in mg/mL, which corresponds numerically to µg/µL.