🇪🇺 Fast delivery (3–5 working days) with GLS ✅ Card or bank transfer 📦 Free shipping from €50
TB500
TB500 5 mg/10mg – Synthetic thymosin beta-4 fragment for research
Peptology Ltd. · Recovery and anabolic peptides
TB500 5mg/10mg – a synthetic peptide corresponding to the actin-binding region (LKKTETQ) of thymosin beta-4 (Tβ4, a 43-amino-acid protein); research peptide for cell-migration and tissue-repair models. Purity 99%+, HPLC/MS report for every batch.
📦 The product is in our EU warehouse – delivery within 3–5 working days.
20,00 € · In stock
- 5mg — 20,00 €
- 10mg — 35,00 €
For research use only / Not a medicine
| Purity | >99% |
|---|---|
| Method | HPLC + LC/MS |
| Laboratory | Analiza Białek |
| Batch | 0226 |
Last updated: 18/10/2026
What is TB-500 (Thymosin Beta-4)?
TB-500 is a synthetic peptide corresponding to the active fragment of thymosin beta-4 (Tβ4) — a natural, 43-amino-acid protein that the body releases at the site of injury. TB-500 contains the key, actin-binding region of Tβ4, responsible for cell migration, tissue repair and anti-inflammatory action. In the research literature it is described as one of the most important regenerative peptides, targeted at healing and rebuilding soft tissues.
TB-500 belongs to a different class than the GH-axis peptides. Molecules such as CJC-1295 or Ipamorelin stimulate the secretion of growth hormone, and IGF-1 LR3 drives hypertrophy. TB-500 acts differently — it regulates actin and directs repair cells to the damaged tissue, supporting angiogenesis and limiting inflammation. That is why in studies it is often combined with BPC-157 as a complementary regenerative pair.
TB-500 is studied in the context of wound healing, repair of tendons, ligaments and muscles, regeneration of cardiac tissue and modulation of inflammation. It is a research peptide — it holds no authorization as a medicinal product. See also the other recovery and anabolic peptides in our category.
In the version offered by Peptology, TB-500 is supplied as a lyophilized peptide with purity above 99% (HPLC/MS), confirmed by independent laboratory analysis (CoA for every batch). It is available in two vial formats — 5 mg and 10 mg — depending on the scale of the research protocol.
Available formats: 5 mg and 10 mg
TB-500 is available in two lyophilized formats that cover different scales of research work — from single in vitro series to longer regenerative protocols with higher peptide consumption.
5 MG
Standard format
Suitable for validation experiments, single in vitro series and shorter regenerative protocols.
10 MG
Enlarged format
For longer research series, tissue-repair models and protocols with higher peptide consumption.
Both formats have identical purity > 99% and the same laboratory certificate for the batch. The choice of size depends on the scale of the protocol, not on the concentration of the active substance.
How TB-500 works — actin regulation and cell migration
In the research literature, TB-500 (thymosin beta-4) is described as a molecule acting through several complementary repair mechanisms. Their common denominator is accelerating the movement of repair cells to the damaged tissue and rebuilding its blood supply.
ACTIN & MIGRATION
Sequesters G-actin, creating a reservoir for the F-actin network — this drives the migration of fibroblasts, endothelial cells and progenitor cells to the site of injury.
ANGIOGENESIS
Supports the formation of new blood vessels — endothelial cell migration and the formation of vascular structures, improving the blood supply of the healing tissue.
ANTI-INFLAMMATORY & ANTIFIBROTIC
Reduces the production of inflammatory cytokines and apoptosis and limits the formation of myofibroblasts — less scarring, more functional repair.
Why the actin mechanism matters
Most tissue regeneration begins with one limiting step — the repair cells must physically reach the site of damage. Thymosin beta-4 regulates actin, the cytoskeleton responsible for cell movement. Thanks to this, TB-500 is associated in studies with faster cell migration, mobilization of stem and progenitor cells, and better blood supply — which together accelerate healing and limit scar formation.
This mechanism is complementary to BPC-157, which acts mainly cytoprotectively and pro-angiogenically — which is why the two peptides are often studied together. This data comes from preclinical studies and does not constitute a claim about the effect of the research-grade product.
Pharmacokinetics and pharmacodynamics of TB-500
TB-500 is a small, well-soluble peptide, which in the published data is described as a molecule with a relatively short plasma half-life but with effects that last longer thanks to redistribution to tissues.
- Plasma half-life: on the order of 2–3 hours (data from animal models after SC/IM administration)
- Distribution: low molecular weight favors good distribution to tissues and redistribution after the plasma phase
- Profile of action: tissue effects last longer than the presence of the peptide in plasma
- Target: actin regulation (G-actin sequestration), migration and repair signaling
- Route of administration in protocols: subcutaneous or intramuscular, per the research protocol
- Schedules: loading and maintenance phases are described in the literature, often in cycles
TB-500 vs BPC-157 vs IGF-1 LR3
The table below compares the three most frequently studied "regenerative" peptides with different mechanisms. TB-500 regulates actin and cell migration, BPC-157 acts cytoprotectively and pro-angiogenically, and IGF-1 LR3 drives growth and hypertrophy. Because their mechanisms are complementary, in studies they are sometimes combined into a "regenerative stack". The data are scientific and comparative in nature.
| TB-500 | BPC-157 | IGF-1 LR3 | |
|---|---|---|---|
| Class | Thymosin beta-4 fragment | Cytoprotective peptide | IGF-1 analog (growth factor) |
| Main mechanism | Actin regulation, cell migration | Cytoprotection, angiogenesis | Protein synthesis, hypertrophy |
| Main research area | Wound healing, soft tissues, tendons | GI tract, tendons, vessels | Muscle growth and regeneration |
| Half-life | ~2–3 h (effects longer) | Short | ~20–30 h |
| Role in the regenerative "stack" | Tissue migration and repair | Protection and blood supply | Anabolism |
The values and properties are approximate and come from the literature on thymosin beta-4 and regenerative peptides. The table is scientific and comparative in nature and does not constitute a direct head-to-head comparison or a claim about the effect of the research-grade product.
Research and clinical data on TB-500 / thymosin beta-4
Most data concern the full thymosin beta-4 (Tβ4) molecule; the TB-500 fragment itself is studied mainly preclinically, but shares the same active region with Tβ4. Below are the most important observations from the literature.
Wound healing — Tβ4's flagship area
Wound healing is the best-documented application of thymosin beta-4 and the only one with data from clinical trials in humans. Numerous independent laboratories reproduced the effects of Tβ4 in various injury models — from skin wounds to soft tissues — associating them with faster cell migration and better blood supply.
Clinical trials of full Tβ4 (RGN-259, RGN-137)
Full thymosin beta-4 was the subject of clinical trials (including phase 2) under the codes RGN-259 (dry eye) and RGN-137 (chronic wounds), as well as in neurorestorative programs. These studies do not concern the TB-500 fragment directly, but share its active region and provide context regarding safety and efficacy.
Soft-tissue and cardiac models
In preclinical models, Tβ4 / TB-500 is studied in the context of the repair of tendons, ligaments and muscles and the regeneration of cardiac tissue after ischemia. The results are associated with the mobilization of progenitor cells, angiogenesis and the limitation of fibrosis.
Note: The information is presented for scientific and informational purposes and is based on the preclinical literature and studies of full thymosin beta-4. It does not constitute medical advice, a recommendation for use or a claim about the effect of the research-grade product offered by Peptology.
TB-500 profile at a glance
- Active fragment of thymosin beta-4 — a regenerative peptide targeted at soft-tissue repair
- Actin regulation and cell migration — accelerates the movement of repair cells to the site of injury
- Supports angiogenesis — rebuilding the blood supply of the healing tissue
- Anti-inflammatory and antifibrotic action — less scarring, more functional repair
- Wound healing with clinical data for full Tβ4 (RGN-259, RGN-137)
- Complementary to BPC-157 — a classic pair in regeneration research
All information provided refers to the preclinical and clinical literature on thymosin beta-4 / TB-500 and should not be interpreted as a promise of effect, an instruction for use or a claim about the effect of the research-grade product offered by Peptology.
Research dosing protocols
Important: The information below describes schedules used in research on TB-500 / thymosin beta-4. It is provided for scientific and informational purposes and does not constitute a recommendation for individual use, medical advice or administration instructions. The research-grade TB-500 offered by Peptology is not a registered drug.
In the research literature, TB-500 is described in schedules with a loading and maintenance phase, administered subcutaneously or intramuscularly:
- Loading phase: more frequent administration in the first weeks of the protocol
- Maintenance phase: less frequent administration sustaining the regenerative effect
- Subcutaneous or intramuscular administration, per the research protocol
- Cyclicity: the literature describes cycles with break periods and monitoring of the response
In regeneration protocols, TB-500 is sometimes combined with BPC-157 (complementary mechanisms) and with GH-axis peptides, like CJC-1295 or IGF-1 LR3. These schedules come from the research literature and do not constitute a recommendation for self-administration.
Side effects and safety
In studies and observations, TB-500 is described as well tolerated, with rare and mild side effects. Most of them are transient and dose-dependent.
Frequently observed
- Mild discomfort at the injection site
- Transient fatigue or drowsiness (first days)
- Slight headache
- A "rush" / warmth sensation after administration
Less frequently described
- Slightly increased appetite
- Transient dizziness
- Local redness
To consider
- Pro-angiogenic action — caution in proliferation contexts
- Hypersensitivity to formula components
- No long-term safety data
Most observed side effects are mild and reversible. As a pro-angiogenic peptide, TB-500 requires caution in contexts related to cell proliferation. This information is scientific in nature and does not constitute an instruction for use.
Storage and reconstitution
TB-500 is supplied as a lyophilized (freeze-dried) powder in a vial. In lyophilized form the peptide is stable at room temperature, protected from light and moisture — refrigeration before reconstitution is not mandatory. After reconstitution, stability depends on the solvent used, with Peptide Matrix Water™ significantly extending the peptide's lifespan.
- Before reconstitution: room temperature, in the original packaging, protected from light and moisture. A refrigerator is not mandatory.
- After reconstitution with Peptide Matrix Water™: stability in the refrigerator +2 °C to +8 °C for up to 60 days under appropriate laboratory conditions — the stabilizing formula extends the peptide's lifespan relative to standard solvents.
- After reconstitution with bacteriostatic or sterile water: stability in the refrigerator +2 °C to +8 °C for up to 28 days under appropriate conditions. Do not refreeze the reconstituted solution.
- Reconstitution: in a laboratory setting, Peptide Matrix Water™, bacteriostatic water or sterile water for injection is usually used, depending on the protocol.
- Volume: maximum 3 mL of solvent (capacity of a standard vial). For a precise calculation of volume and units, use the peptide calculator.
- Homogenization: add the solvent slowly down the vial wall, without vigorous shaking — peptide bonds are sensitive to mechanical stress. Swirl gently, do not shake.
Laboratory tip: With your order, you can add Peptide Matrix Water™ to the cart — a stabilizing solution designed for working with reconstituted research peptides. More in the article Bacteriostatic water vs acetic acid vs matrix water.
Frequently asked questions about TB-500
What exactly is TB-500?
It is a synthetic peptide corresponding to the active, actin-binding region of thymosin beta-4 — a protein released by the body after injury. It supports cell migration, angiogenesis and anti-inflammatory action, accelerating tissue repair. The results come from the research literature.
How does TB-500 differ from BPC-157?
Both are regenerative peptides, but they act differently. TB-500 regulates actin and cell migration (the movement of repair cells to the injury), while BPC-157 acts mainly cytoprotectively and pro-angiogenically. Their mechanisms are complementary, which is why they are often combined into a pair in studies.
Is TB-500 the same as thymosin beta-4?
TB-500 is a synthetic peptide corresponding to the active fragment of thymosin beta-4 (Tβ4). It shares the same actin-binding region with full Tβ4, which is why data from studies on Tβ4 are an important context, although they do not concern the TB-500 fragment directly.
Can it be combined with other peptides?
Yes — in studies it is classically combined with BPC-157 for a complementary regenerative effect, and also with GH-axis peptides (CJC-1295, Ipamorelin) or with IGF-1 LR3.
Which format to choose — 5 or 10 mg?
Both have identical purity > 99% and the same batch certificate. 5 mg works well for shorter protocols and single series, and 10 mg — for longer tissue-repair models with higher peptide consumption.
How is TB-500 stored and reconstituted?
The lyophilized vial is stored at room temperature, protected from light and moisture. After reconstitution, stability depends on the solvent: with Peptide Matrix Water™ — up to 60 days in the refrigerator +2 °C to +8 °C; with bacteriostatic or sterile water — up to 28 days. Avoid refreezing.
Where to buy TB-500 for research?
Peptology offers TB-500 (5 / 10 mg) as a research peptide with confirmed purity above 99% (HPLC/MS) and independent laboratory analysis for every batch. Fast shipping, discreet packaging and certificates of purity with the product. For scientific background, also see the article Peptides — what they are, how they work, main categories and safety profile.
Related peptides in the Peptology catalog
Researchers working with TB-500 often also consider other regenerative peptides and GH-axis tools that complement its action. Each of the products below has a certificate of purity above 99%:
BPC-157
A complementary regenerative peptide
IGF-1 LR3
Anabolic growth factor
CJC-1295 without DAC
GHRH analog (GH axis)
Ipamorelin
Selective GH secretagogue
CJC-1295 + Ipamorelin
Ready-made GH-axis complex
Peptide Matrix Water™
Stabilizing solution for reconstitution
See the full catalog: Recovery and anabolic peptides · Solutions for peptides · All peptides · Blog on peptide research
Research warning: TB-500 (thymosin beta-4) is offered exclusively for scientific research and in vitro purposes. It is not a medicinal product, is not approved for use in humans or animals, and does not replace medical advice. All information comes from the preclinical and clinical literature on thymosin beta-4 and is provided for scientific and informational purposes; it does not constitute a claim about the effect of the research-grade product offered by Peptology.
Molecule identity
| CAS number | 885340-08-9 |
|---|---|
| Molecular weight | 889.0 g/mol |
| Molecular formula | C38H68N10O14 |
| Class | Thymosin beta-4 fragment |
| Form | Lyophilised powder in a glass vial |
| Storage | Lyophilisate: 2–8 °C, dry and protected from light · after reconstitution: 2–8 °C |
Laboratory reconstitution
Laboratory protocol — reconstitution, research protocols, storage
Standardized protocol applicable to all research peptides in the Peptology catalogue. To calculate the solution concentration (mg/mL) for each vial, use the peptide reconstitution calculator.
60-second protocol
1. SOLVENT
2. RECONSTITUTION
Add the solvent down the side of the vial, without shaking, wait 10–15 min.
3. CALCULATION
Calculator → concentration mg/mL
4. STORAGE
+2 °C to +8 °C, up to 60 days with Peptide Matrix Water
Quick reference
| Parameter | Value |
|---|---|
| Form | Lyophilized powder |
| Solvent | Peptide Matrix Water |
| Reconstitution volume | 1–3 mL — maximum 3 mL (capacity of the standard vial) |
| Storage before reconstitution | Room temperature, protected from direct light (refrigeration not required) |
| After reconstitution | Up to 60 days at +2 °C to +8 °C with Peptide Matrix Water |
What you need
Precision pipette
Calibrated, 100–1000 µL — for accurately measuring the volume of solvent added to the vial.
70% alcohol swabs
Two swabs — to disinfect the peptide vial and the solvent vial.
Stabilized solvent for lyophilized peptides. Why this one.
Five rules for reconstitution
| ✅ Correct | ❌ Mistake |
|---|---|
| Disinfect the vial caps and the work surface with 70% alcohol; wear gloves | Working without disinfection |
| Add the solvent slowly along the vial wall | Direct jet onto the powder — foaming and denaturation |
| Gentle rolling between the palms | Shaking — foam, which denatures peptides |
| 10–15 minutes waiting at room temperature | Mechanical mixing or heating |
| Visual check: clear, particle-free | Working with a cloudy or discolored solution |
Calculating concentration
⚠️ Maximum 3 mL solvent. The standard vial does not accommodate larger volumes — do not exceed during reconstitution.
- Concentration (mg/mL) = mg in the vial ÷ mL of solvent
- Volume per sample (mL) = amount per sample (mg) ÷ concentration (mg/mL)
- 1 mg/mL = 1 µg/µL · 1 mL = 1000 µL
Reconstitution per vial — solvent: Peptide Matrix Water. Concentration after adding the solvent (1 mg/mL = 1 µg/µL):
| Vial | + 1 mL | + 2 mL | + 3 mL |
|---|---|---|---|
| 5 mg | 5 mg/mL | 2.5 mg/mL | 1.67 mg/mL |
| 10 mg | 10 mg/mL | 5 mg/mL | 3.33 mg/mL |
👉 For any vial and solvent volume — use the calculator. It calculates the concentration (mg/mL, µg/µL) and the volume per sample (mL, µL).
Storage
BEFORE RECONSTITUTION
Room temperature, protected from direct light, in original packaging. Refrigerator not required.
AFTER RECONSTITUTION
+2 °C to +8 °C, up to 60 days with Peptide Matrix Water. Discard any remainder after this period.
FREEZING
Not recommended. Freeze/thaw cycles degrade peptide structure.
Four mistakes to avoid
1. Shaking "to dissolve faster"
Foam = denaturation. Only gentle rolling between the palms.
2. Inappropriate solvent
For peptide stability use only sterile, specially formulated solvent — recommended Peptide Matrix Water.
3. Ignoring slight turbidity
Not fully clear = the sample does not meet the criteria for use.
4. Taking it out of the refrigerator "for an hour–two"
Short exposures add up and compromise stability.
Research status: All Peptology products are intended for laboratory and research use only. They are not medicinal products, not approved for human or veterinary use and do not substitute medical advice. The described protocol is standard laboratory practice for working with lyophilized peptides.
Shipping information
🚚 Shipping information
-
Products in our warehouse – orders placed by 15:00 on working days are dispatched the same day, later orders on the next working day.
-
Delivery with GLS to your address in 3–5 working days.
-
Shipping costs €5 and is free from €50.
Frequently Asked Questions (FAQ)
In what form is TB500 supplied and how should it be stored?
It is supplied as a lyophilized (freeze-dried) powder in a sealed vial. Store the unopened vial dry and protected from light, ideally at 2–8 °C. After laboratory reconstitution, keep the solution at 2–8 °C and use it within the period stated in the “Laboratory reconstitution” tab (up to 60 days with Peptide Matrix Water).
What purity does TB500 have and how is it tested?
Every batch has a purity of 99% or higher, confirmed by HPLC and mass spectrometry (MS) and documented in a certificate of analysis (CoA). The report for the current batch is shown in the “Laboratory Analysis” tab.
Is a prescription required?
No. TB500 is intended for research purposes and does not require a prescription.
Where can I buy TB500 in Europe and what does it cost?
You can buy TB500 in Europe online from Peptology, from 20,00 €. Delivery with GLS in 3–5 working days. Shipping is free from 50,00 €. Payment by card. Purity 99%+, with an HPLC report for every batch.
Laboratory Analysis
TB500 - 5mg - Batch - 0226 - Purity
TB500 - 10mg - Batch - 0226 - Purity
